Fig. 6

Images showing Calr immunostaining in NTD aged 28 (A), 44 (B), and 174 (C, D) wk and in DS at 32 (E), 44 (F), and 196 (G, H) wk. No positive cells were observed in 28 wk NTD (A), while small neurons displayed staining at 44 wk NTD in layers II/III (B). In 174 wk NTD, large bipolar fusiform cells were observed in layers II/III (C) and layer V (D). A 32 wk DS case displayed small Calr-ir cells in layers II/III (E). Note that the cellular processes stained more intensely in layers II/III at 44 wk (F). In a 196 wk DS case, fusiform Calr-ir cells were observed in layer III (G) and layers V/VI (H). Images showing the absence of Parv immunoreactivity in FC SG and IG layers in 28 and 174 wk NTD (I–L) and the youngest 31 wk DS cases (M–N). Small and intensely stained Parv-ir cells were seen in layers V/VI in the oldest 196 wk DS case (O, P). Scale bars = 50 µm