Fig. 3

Readout of total aSyn on RPA is validated by two specific immunoassay platforms. A, B: High correlations between alphaLISA® and Elecsys® platforms in detergent-soluble (A) and detergent-insoluble fractions (B) in all brain regions. Concentrations on x- and y-axes are both expressed in µg/g wet tissue. Note the substantially lower levels for total aSyn in detergent-soluble fractions of the Substantia nigra (SN) compared to the hippocampus (Hip) and putamen (Put). C, D quantitative measurements of total aSyn levels as measured by alphaLISA® show high correlations with the RPA signal intensities over all samples, both in detergent-soluble (C) and detergent-insoluble (D) fractions. Values represent Spearman’s correlation coefficients. ***p < 0.001