Fig. 1

Somatic alterations in multi-region tumor samples acquired from DIPG patients treated with a PDGFR inhibitor. A Somatic alterations affecting known DIPG driver genes in 49 tumor samples from 11 DIPG patients treated with crenolanib under stratum A (for newly diagnosed DIPG) or stratum B (for progressive DIPG). Each column represents a single tumor sample from a tumor region in the numeric order of A1, A2, etc.; tumor samples from the same patient are grouped together and labeled by patient ID at the top. Patient and sample metadata are shown at the top while somatic alterations colored by their variant type are shown at the bottom. Within the same patient, presence of a different mutation in the same gene is marked by an asterisk “*” while admixture of multiple mutations in the same sample is marked by “#”. Treatment duration varies broadly ranging from 15 to 658 days. B Somatic SNV/indel burden of each patient stratified by their presence in multi-region tumor samples including truncal (detected in all samples), shared (detected in multiple but not all samples) and private mutations (detected in a single sample) shown as the count (top panel) and percentage (bottom panel) of mutations in each patient. C Somatic arm-level or whole-chromosome CNVs of each patient shown in the same style as B. Only samples profiled from SNP arrays are included and BAF-adjusted CNVs were used for plotting