Fig. 5

SNCA regulates the expression of genes identified in the CK2α’ mediated RNA signature. A, SNCA and DGEs connection by IPA network analysis. Purple asterisks denote synaptic function. B, RT-qPCR in Q7 and Q111 cells (n = 6–8 experiments). C–E, siRNA knockdown of SNCA (siSNCA) for 24 h in STHdh Q7 (D) and Q111 cells (E), and RT-qPCR for SNCA (C) and SNCA putative gene targets (D, E). Data were normalized with GAPDH and relativized to non-targeting control siRNA-treated cells (Scr.), (n = 5 experiments). Four data points fell in the axis break for Slc30a3. F, Immunoblotting for α-syn, GFP and GAPDH in Q7 and Q111 cells after plasmid transfections. Q7 and Q111 cells were transfected with pEGFP (control) or α-syn-GFP overexpression (OE) and harvested 24 h after transfection. G, H, RT-qPCR for Q7 (G) and Q111 cells (h) transfected with control or a-syn-GFP. Data were normalized with GAPDH and relativized to non-targeting control pEGFP-treated cells, (n = 3 experiments). Data are mean ± SEM with significance determined by Welch’s t-test