Fig. 2

CK2α’ haploinsufficiency increased frequency of striatal AMPA-mediated miniature excitatory postsynaptic currents (mEPSC) in zQ175 mice. a, b, Representative images show the labeling (A) and quantification (B) of CK2α’ in striatal MSNs immunostained for Ctip2, a specific MSN marker in WT, zQ175 and zQ175:CK2α’ ^(±) mice at 12 months of age (n = 7–8 mice/genotype, 6 images averaged/mouse). Scale bar, 50 µm. C, Image shows whole-cell patch-clamp recording diagram in acute dorsolateral striatum slices, where Ctip2 labeled MSNs from 12-month-old mice. Scale bar 500 µm, Ctr: Cortex; Str: Striatum. D, Input–output curve (WT, n = 8 cells from 3 mice; zQ175, n = 9 cells from 4 mice; zQ175:CK2α’^(±) n = 13 cells from 4 mice). Representative traces are shown in the top inset. E, Short-term potentiation measured via paired-pulse facilitation (WT, n = 8 cells from 3 mice; zQ175, n = 9 cells from 4 mice; zQ175:CK2α’^(±) n = 11 cells from 4 mice). Representative traces of two consecutive stimuli delivered at 25 ms time intervals are shown in the top inset. F, Short-term depression analyzed through synaptic fatigue (WT, n = 7 cells from 3 mice; zQ175, n = 9 cells from 4 mice; zQ175:CK2α’^(±) n = 12 cells from 4 mice). Representative traces are shown in the top inset. Values were analyzed using two-way ANOVA with Tukey’s post-hoc analysis. G, H, Recordings of mini excitatory postsynaptic currents (mEPSCs). Amplitude (in pA; left panel) (G) and frequency (in Hz; right panel) (h) were analyzed (WT, n = 10 cells from 3 mice; zQ175, n = 9 cells from 4 mice; zQ175:CK2α’^(±) n = 12 cells from 4 mice). I, Representative mEPSC traces. Values were analyzed using one-way ANOVA with Dunn´s post-hoc analysis. p values < 0.05 are indicated. Error bars represent mean ± SEM