Fig. 1
CK2α’ levels progressively increase in striatum of zQ175 and correlate with HTT aggregation and NeuN depletion. A–F, Immunostaining and quantification of HTT puncta detected with anti-HTT EM48 antibody (n = 6 mice/genotype, 6 images averaged/mouse) (A, B), NeuN+ cells (n = 3 mice/genotype, 15 images averaged/mouse) (C, D) and CK2α’ levels (n = 6 mice/genotype, 6 images averaged/mouse) (E, F) in zQ175 compared with WT mice at 3, 6, 12 and 22 months. G, CK2α’ mRNA levels analyzed by RT-qPCR in striatum and cortex of 6-month-old mice. Data was normalized to GAPDH and WT striatum (n = 3 mice/genotype,). H, Linear regression analysis between CK2α’ levels and HTT puncta, and I, between CK2α’ levels and number of NeuN+ cells in zQ175 mice. The Pearson correlation coefficient (ρ) and R2 are indicated. Data are mean ± SEM with significance determined by one-way ANOVA with Dunnett’s post-hoc test in B, mean ± SD with significance determined by one-way ANOVA Dunnett’s post-hoc test in F and two-way ANOVA withTukey’s post-hoc test in D and G. p-values < 0.05 are indicated. n.s = not significant. Scale bar, 50 µm