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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: The distribution and function of GDE2, a regulator of spinal motor neuron survival, are disrupted in Amyotrophic Lateral Sclerosis

Fig. 2

Loss of GDE2 exacerbates SOD1G93A pathology. A-L Immunostaining of cytoskeletal markers A-H; and astrogliosis (GFAP) and microgliosis (Iba1) markers I-L in transverse sections of 14-week lumbar spinal cord. Note that images in K and L were increased in contrast compared with I and J to show background staining levels in absence of neuroinflammation. Arrowheads in B and F show increased cytoskeletal inclusions in the Gde2+/−;SOD1G93A spinal cord. Insets shows somal inclusions. Arrows in J highlight exacerbated microgliosis. No cytoskeleton accumulations, astrogliosis or microgliosis are evident in the Gde2+/−;SOD1WT and Gde2+/+;SOD1WT controls. M, N Graphs show increased peripherin (*p = 0.04), phospho-NFH (*p = 0.03) and microgliosis (*p < 0.01) in Gde2+/−;SOD1G93A spinal cords compared with Gde2+/+;SOD1G93A controls. Astrocyte area fraction is unchanged (p = 0.13). Student’s t test, n = 4 Gde2+/+;SOD1G93A, 3 Gde2+/−;SOD1G93A, 3 Gde2+/+;SOD1WT, 4 Gde2+/−;SOD1WT. Scale bar = 50 μm. Graphs represent mean ± SEM

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