Fig. 2

Representative multiplex immunofluorescence images of cored (2a) and diffuse (2b) plaques, and correlations between fluorescence and immunohistochemical staining of A\(\upbeta\) with antibodies 6E10 and 4G8 (2c). Scale bar 20um for all images. a multiplex immunofluorescence of a cored plaque stained for Aβ (6E10 primary antibody with AF488 secondary antibody, green), GFAP (AF555 secondary antibody, magenta), CD68 (AF647 secondary antibody, red); with merged channels in the bottom right of the panel. b multiplex immunofluorescence of a diffuse plaque stained for Aβ (6E10 primary antibody with AF488 secondary antibody, green), GFAP (AF555 secondary antibody, magenta), and Iba1 (AF647 secondary antibody, red) with merged channels in the bottom right of the panel. 2c: Examples of cored and diffuse Aβ plaques detected by immunofluorescence with primary antibody 6E10 (row 1) and diaminobenzidene immunohistochemistry with primary antibody 4G8 (row 2)