Fig. 4
PFF-induced alteration of mitochondrial protein levels. A Representative western blot images of mitochondrial proteins VDAC1, COX-IV, Tomm-20, and Ndusf4 in homogenized cortex samples from monomer- and PFF-injected mice 3 months p.i. B Increased levels of VDAC1 in the contralateral compared to ipsilateral hemisphere of PFF-injected mice (Two-way ANOVA, main effect of PFF, F (1,28) = 6.355; ipsilateral vs contralateral: PFF, Sidak’s multiple comparison’s p = 0.0424; N = 8 monomer, 8 PFF) which was maintained when the contralateral hemispheres were compared between monomer- and PFF-injected mice (Monomer vs PFF: contralateral, Sidak’s multiple comparison’s p = 0.112). C COX-IV levels showed a main effect of PFF injection (Two-way ANOVA main PFF effect, F (1,28) = 7.425, p = 0.0110). No changes were detected in levels of Tomm-20 (D) or Ndusf4 (E) via Two-way ANOVA or multiple comparisons. F Representative western blot images of mitochondrial membrane proteins VDAC1, COX-IV, Tomm-20, and Ndusf4 in monomer- and PFF-injected mice 6 months p.i. G No change in VDAC1 levels. H COX-IV levels were increased in ipsilateral PFF-injected mice compared to monomer (Two-way ANOVA, Sidak’s multiple comparison’s p = 0.0299, N = 9 monomer, 9 PFF). No change detected in Tomm-20 (I) or Ndusf4 (J) levels. GAPDH was used as a loading/normalization control