Fig. 1

Fibril generation, quality control, and experimental timeline. A Flow chart of procedure used for fibril generation and quality control analysis. B Cup-horn sonicator used to sonicate fibrils. C Electron micrograph of sonicated PFFs diluted 1:300 in PBS and spotted on a formvar-carbon coated EM grid. Scale bar is 100 nm. D Histogram showing the length distribution of sonicated particles measured from the EM micrographs. Fibril lengths were generally uniform with a median length of 29.5 nm. E Histogram of radii of sonicated PFFs measured via dynamic light scattering (median 13.5 nm). F Schematic of intrastriatal injection of 2 μl of either monomer or PFFs followed by euthanasia and harvesting brain for analysis either 3 or 6 months post injection. Ctx: Cortex, Str: Striatum, Thal: Thalamus, vMB: Ventral Midbrain