Fig. 3
Inactivation of FGFR4 reduces proliferative capacity and promotes cell death. A Schemes of the kinase domain-truncated, CFP-coupled tFGFR4 (left) and of the point-mutated (mut), kinase-dead FGFR4-KD(K504M) (right), GFP-coupled proteins are shown. (B + F) Clonogenicity upon tFGFR4 B, FGFR4-KD(K504M) (F) compared to GFP (B + F, set to 1) transduction was analyzed in endogenously FGFR4high (BTL1376, BTL1528) or FGFR4low (U251-MG, BTL1529, BTL53) cell models (mean ± SD from three experiments). C Sphere formation potential of GSC models upon tFGFR4 or GFP transduction (mean ± 25%CI). D Survival/proliferation capacities over time are shown for tFGFR4- and GFP-transduced GBM models. For each model, one representative out of three experiments in duplicates is depicted. E Cell death induction upon tFGFR4 or GFP transduction in GBM models after 3 days. Annexin/PI staining was analyzed by flow cytometry. Significance levels were calculated comparing tFGFR4- to GFP-transduced cells on the respective living or dead fraction. Statistical analyses: 2-way ANOVA/Bonferroni correction (mean ± SD) (B,D,E,F); Student’s t-tests (C). *p < 0.05, **p < 0.01, ***p < 0.001, n.s. = not significant