Fig. 7

Dual-channel longitudinal in vivo miniscope imaging of endothelial cells and new vessel sprouting after cortical injury. The spatiotemporal pattern of ECs expressing tdTomato in a Tie2-Cre; Ai9 mouse was imaged daily at the same field of view in the injured cortex during the first 9 dpi. Over the course of the first 8 dpi (A–F), there is clear clustering of ECs in initial non-perfused microvessels, evidenced by intense fluorescent patchy regions in these vessels. Red arrows (C–E) point at a putative tip cell cluster that continues to migrate with increasing vessel length. The blue arrow E points to a filopodium emanating from the tip of the new micro-vessel. G and I illustrate a functionally perfused microvessel at 8 dpi, visualized with FITC (green) fluorescent-labeled dextran for blood flow, which corresponds to the vessel (white arrows in F and G). H and I at 9 dpi show increasing numbers of functionally perfused vessels. The arrows and arrowheads point to alignment features