Table 4 Morphometry of NMJs from confocal microscopic images
From: Impaired signaling for neuromuscular synaptic maintenance is a feature of Motor Neuron Disease
Morphological variable | non-MND n = 5 mean ± SD | MND n = 3 Mean ± SD | P value un-paired t test |
|---|---|---|---|
Primary Motor axon thickness (μm) | 1.546 ± 0.5 | 1.257 ± 0.7 | n. s. P = 0.44 |
Motor axon terminal sprout thickness (μm) | 0.926 ± 0.4 | 0.73 ± 0.4 | n.s. P = 0.49 |
Motor Nerve terminal Bouton size (μm2) | 8.03 ± 1.7 | 5.01 ± 0.8 | * P = 0.034 |
Total NT area (μm2) | 74.33 ± 10.3 | 36.71 ± 12.2 | ** P = 0.0033 |
AChR cluster patch size (μm2) | 13.82 ± 2.2 | 18.82 ± 7.9 | n.s. P = 0.36 |
Total AChR area (μm2) | 126.5 ± 17.9 | 138.0 ± 26.9 | n.s. P = 0.44 |
% of Motor nerve terminal to AChR overlap | 60.14% ± 2.3 | 29.47% ± 7.1 | *** P = 0.0001 |