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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Impaired signaling for neuromuscular synaptic maintenance is a feature of Motor Neuron Disease

Fig. 4

Differentiation of H9 human embryonic stem cells in culture. A–E Quantification of mRNAs encoding Pax6 (A), Olig2 (B), HB9 (C), ChAT (D) and Islet-1 (E) (n = 3). F Sample visual field of differentiated H9 cells labeled for ChAT, Islet-1, and nuclei (DAPI). Lower right panel shows the merge of ChAT and Islet-1 fluorescence. Scale bar for F = 100 μm. G A time series showing the calcium flux in differentiated neurons after addition of 50 mM KCl at time zero. Scale bar for G = 160 μm. The inset graph shows the time course of intracellular Ca2+ mobilization revealed by Ca2+ sensor fluorescence GCaMP6f. ΔF/F0 represents the fluorescence intensity relative to the initial fluorescence intensity. Data presented in A–E are means ± SDs, expression data analyzed using one-way ANOVA with Bonferroni’s multiple comparisons test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001

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