Fig. 3

MuSK staining is more diffuse relative to the AChR-rich motor endplate. A Compares the staining areas for acetylcholine receptors (AChRs) and MuSK at the motor endplate from a non-MND (Con-2) donor, and from an MND patient (MND-3). At the motor endplate of MND-3, MuSK staining occupied a larger area compared to the AChR area. By contrast, the MuSK area was a closer match to AChR at the Con-2 motor endplate. B Shows the relative pixel area occupied by AChR and MuSK staining per motor endplate from non-MND donors (Con-1, -2, and 4) and MND patients (MND-1, -3 and -5). At non-MND motor endplates, there appeared to be tight co-localization of AChR and MuSK staining as depicted by the closeness of their respective AChR and MuSK data points. By contrast at MND motor endplates, MuSK occupied a larger area of staining compared to AChR area. Scale bar = 20 μm