Fig. 12

Levels of n-agrin effector proteins are altered in muscle cultures from MND patients. A Sample immunoblot comparing the relative intensity of MuSK, Caveolin-3 and Dok7 bands in myotube cultures derived from 4–5 non-MND donors and 6 MND patients. Antibodies revealed bands of the expected molecular weight for MuSK (~ 97 kDa), Caveolin-3 (~ 20 kDa) and Dok7 (~ 55 kDa). Each band was normalized to a tubulin loading control band from the same lane (shown beneath). Comparison of the normalized band intensities for B MuSK, C Caveolin-3, and D Dok7. E Sample immunoblot for LRP4 (~ 212 kDa). F Compares the normalized band intensities for LRP4 in non-MND and MND myotube cultures. Symbols each represent cultures from an individual donor (blue and yellow data). G Key showing the source of the samples run in the indicated lanes of the immunoblots. All data presented as mean ± SD and were analyzed using unpaired t-tests where *p ≤ 0.05 and **p ≤ 0.01