Fig. 10

Sample images showing AChR clusters on myotubes cultured from non-MND and MND muscle before and after n-agrin treatment. A Sample field of desmin-positive myotubes cultured from muscle of non-MND patient Con-6, prior to n-agrin treatment. Small and occasional large AChR clusters are evident (yellow and white arrows respectively). A′ and A″ These AChR clusters at higher magnification. B Sample field of desmin-positive myotubes from Con-6 after n-agrin treatment. Large and small AChR clusters appear more numerous after n-agrin treatment (arrows and magnified views are shown in B′ and B″). C, D Comparable sample fields of myotubes cultured from MND-8 (C) before and (D) after n-agrin treatment. Only small AChR clusters are visible both before and after n-agrin treatment (white arrows in C and D and their high-powered views: C′–C″ no n-agrin, and D′–D″ plus n-agrin). Scale bars = 50 µm