Fig. 1

Accelerated induction of ptau pathology in PS/E3H mice seeded with K18-tau aggregates. K18-tau fibrils were injected into the left hippocampus of 2.5-month-old mice and aged for 5 months. 7.5-month-old mice were then analyzed using AT8 antibody. Representative images from the hippocampus and cortex of K18-tau aggregate injected hemisphere (ipsilateral, ‘IPSI’) and uninjected hemisphere (contralateral, ‘CONTRA’) showing ptau pathology in PS19 mice homozygous for APOE alleles (B6N2 generation) or PS19 mice carrying murine Apoe (a). Quantification of the antibody immunostaining is presented as % immunoreactivity in the cortex (Ctx) or hippocampus (Hpc) from ipsilateral and contralateral hemispheres around the injection site (b). Boxes in whole brain panel indicate selected areas used for high power zoomed panels. n = 9–12 mice/group. 1-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar: 3 mm (whole brain); 100 µm (hippocampus and cortex)