Fig. 6
ZIKV infection induces glial activation in the CNS of P7 AIRlow neonates. Representative sections from P7 (a, c, e and g) IgR and (b, d, f and h) AIRlow mice demonstrating (a–d) GFAP and (e–h) Iba1 immunofluorescence labeling in the cortex and cerebellum. Specific labeling from each antibody is shown in magenta and cell nuclei (Hoechst) are shown in grey for counterstain. Green boxes in (g) and (h) correspond to high magnification insets to demonstration cellular morphology. P7 neonatal whole brain from naïve, IgR and AIRlow pups was evaluated for RNA expression of glial-specific genes including (i) Gfap, (j) Aif1 (Iba1) and (k) Gpr84 by qRT-PCR with specific primers. Each symbol indicates an individual brain. The mean of the individually plotted data points for each group is represented by the horizontal black bars. A One-way ANOVA was used to compare RNA expression of (i) Gfap (F2, 20 = 44.50, p < 0.0001), (j) Aif1 (F2, 20 = 76.88, p < 0.0001) and (k) Gpr84 (F2, 20 = 103.2, p < 0.0001) from samples from each group and a Tukey Multiple Comparisons Test was used to determine significance between groups. ****p < 0.0001, indicates significance between groups