Skip to main content
Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Zika virus vertical transmission in interferon receptor1-antagonized Rag1−/− mice results in postnatal brain abnormalities and clinical disease

Fig. 5

ZIKV infection increases cell death in the CNS of P7 AIRlow neonates primarily in the cerebellum, cortex and spinal cord. Representative H&E-stained sections from P7 (a, c) naïve and (b, d) AIRlow cerebellum and spinal cord respectively. Degenerating cerebellar neurons (b, black arrows) and axonal degeneration (d, black arrows) were observed in AIRlow mice. The scale bar in (b) applies to (a) and the bar in (d) applies to (c). Representative immunofluorescence labeled CNS sections from P7 (eg) IgR and (hj) AIRlow pups demonstrate ZIKV NS5 (green) and active-Caspase 3 (magenta) positive cells in cortex, hippocampus and cerebellum. Nuclei are shown in grey. Images of active-Caspase 3 and nuclei staining only in AIRlow mice are shown in (km). Red arrows and box insets in (eg) indicate baseline active-Caspase 3 labeling in control animals. Red boxes in (hj) correspond to insets in each specific brain region. Yellow arrows indicate ZIKV/active-Caspase 3 dual positive cells while orange arrows indicate active-Caspase 3 only cells. The scale bar in (g) applies to (em)

Back to article page