Fig. 4

mRNA expression of microcephaly-related genes is moderately increased and cerebellar progenitor development is altered in AIR^low mice. P7 neonatal whole brain from naïve, IgR and AIR^low pups was evaluated for RNA expression of neurodevelopmental genes including a Mcph1, b Aspm, c CASC5, d WDR62, e CDK5, and f Cenpj by qRT-PCR with specific primers. Each symbol indicates an individual brain. The mean of the individually plotted data points for each group is represented by the horizontal black bars. A One-way ANOVA was used to compare RNA expression of a Mcph1 (F_{2, 20} = 5.98, p = 0.0092), b Aspm (F_{2, 20} = 5.66, p = 0.0113), c CASC5 (F_{2, 20} = 3.99, p = 0.0349), d WDR62 (F_{2, 20} = 17.79, p < 0.0001), e CDK5 (F_{2, 20} = 7.21, p = 0.0044), and f Cenpj (F_{2, 20} = 7.33, p = 0.0041) from samples from each group and a Tukey Multiple Comparisons Test was used to determine significance between groups. *p < 0.05, **p < 0.01, ****p < 0.0001, indicate significance between groups. n.s. = not significant. The (g) thickness of the EGL at the internal fold of the primary cerebellar fissure was measured in (h) 3 naïve, (i) 9 IgR and (j) 6 AIR^low pups at P7. In (g), each symbol represents the mean measurement from all sections for each hemisphere of each animal. The mean of the individually plotted data points for each group is represented by the horizontal black bars. A One-way ANOVA was used to compare EGL thickness (g, F_{2, 35} = 13.02, p < 0.0001) from samples from each group with a Tukey Multiple Comparisons Test to determine significance between groups. *p < 0.05, **p < 0.01, ****p < 0.0001 indicate significance between groups. In (h–j) the black line indicates the measured thickness of the EGL and actual recorded measurements are shown in the top right. The scale bar in (h) applies also to (i and j)