Fig. 1

Mical precipitates global changes in the interactome of Tau. a Volcano plot of the log2 fold change versus the − log10 (p-value) representing the differential interactions of Tau upon co-overexpression with Mical. Proteins involved in microtubule cytoskeleton organization are highlighted in green, Actin cytoskeleton organization in blue, oxidation–reduction processes in red and synaptic transmission in cyan. b Endogenous microtubules (upper panel), preformed bovine microtubules (middle panel) or phalloidin-bound F-Actin (lower panel) have been isolated from lysates expressing under the elav^C155-GAL4 driver the hTau^0N4R transgene alone or upon Mical up and downregulation. p: pellet and s: supernatant fractions were analyzed by western blotting. Stars indicate significantly altered levels of precipitated Tau upon modulation of Mical levels compared to Tau expressed alone. c Proteins from adult heads following panneuronal expression of the indicated transgenes were sequentially extracted with RIPA buffer and 70% FA and probed for Tau (5A6). Alternatively, aqueous soluble and SDS soluble fractions were probed for Tau using Syntaxin (Syx) as loading control. The bars represent the mean ± SEM relative sol/ins ratios of Tau upon Mical up or down-regulation, over that of Tau alone. Stars indicate significantly altered solubility ratios