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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Neutrophil-vascular interactions drive myeloperoxidase accumulation in the brain in Alzheimer’s disease

Fig. 4

Identification of NETs within blood vessels in human Alzheimer’s disease. AD issue microarrays from the middle temporal gyrus (N = 45–46 per condition) were repeatedly immunostained, imaged, and stripped to build up a multiplexed panel of antibody labelling on the same section. This panel included the specific marker for NETs CitH3, as well as neutrophil/NET markers MPO and S100A8. a Representative images of all stains and insets of b NETs and c MPO-positive microglia. Scale bar = 250 μm, inset = 100 μm. d Quantification of the number of CitH3/MPO/S100A8-positive NETs in AD and control cores. P-values represent results of an two-tailed Student’s T-test. The proportion of e MPO/S100A8-positive neutrophils positive for CitH3, f localised in blood vessels, and g cases positive for NETs in AD and control

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