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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Neutrophil-vascular interactions drive myeloperoxidase accumulation in the brain in Alzheimer’s disease

Fig. 2

MPO accumulation in Alzheimer’s disease and APP/PS1 mice is driven by neutrophil accumulation. a ELISA was performed in neurologically normal and Alzheimer’s disease brains for the myeloperoxidase and calprotectin (S100A8/9 heterodimer). N = 7–13. P-values represent results of a Pearson’s correlation test. b FFPE-embedded human brain sections from the middle temporal gyrus (N = 3–6 per condition) were immunostained for MPO and S100A8. Correlation between MPO and S100A8 positive cells in independent sections. Values represent results of a Pearson’s correlation test. c Image analysis was used to identify cells as S100A8-positive, MPO-positive, or co-labelled. Venn diagram of overlap between S100A8 and MPO. Human brain tissue microarrays from the middle temporal gyrus (N = 21–28 per condition) were labelled with the vascular marker lectin and MPO. d Representative images and e quantification of the localisation of MPO within large (> 12 μm diameter) and small (< 12 μm diameter) lectin-positive vessels. Scale bar = 100 μm. Values represent results of a two-way ANOVA. f Quantification of the percentage of total MPO load present in blood vessels in AD and control brains. Wild-type or APP/PS1 mouse brains at 4 and 12 months of age were labelled for vascular marker tomato lectin (LEL) and neutrophil markers (MPO/S100A8). g Representative images of neutrophils associated with BBB leakage product hemoglobin in AD brains. Scale = 100 μm. h Representative images of MPO and S100A8-positive neutrophils in 12 month-old APP/PS1 vasculature. Scale bar = 5 mm, inset = 100 μm

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