Fig. 1

Cerebral accumulation of neutrophils in APP/PS1 mice and human Alzheimer’s disease. Human brain tissue microarrays (N = 21–46 per condition) of middle temporal gyrus were stained for neutrophil markers a MPO (A0398), b MPO (ab1191060), c MPO (AF3667), d CD66B, and e S100A8. Scale = 250 μm, inset = 25 μm. P-values represent results of an two-tailed Student’s T-test.Brain sections from APP/PS1 or wild type mice were taken at 4 and 12 months, and stained for the neutrophil markers S100A8 and MPO. f Representative images and g analysis of neutrophil abundance in mouse brains. Scale = 100 μm. P-values represent results of a two-way ANOVA. The abundance and activity of neutrophil markers h, i MPO and j calprotectin in human brain tissue lysates was measured by ELISA (N = 7 – 13 per condition). P-values represent results of an two-tailed Student’s T-test