Expression of AQP4 and GFAP in the micro vesicular fraction of the CSF. a Schematic representation of vesicular extraction. b Expression of AQP4 is specifically found in the P2 and P3 fractions and is absent in the total protein fraction. TTR (14KD) was used as a load control since its expression was found in all fractions, including total protein obtained from supernatant 3. c Expression of GFAP and AQP4 in vesicular fractions from pellet 2 and pellet 3. No expression was found in total protein from supernatant 3. d Graphic representation of the colocalization between AQP4 and GFAP, with few events that present only AQP4 or only GFAP. e Graphic representation of fluorescence intensity showing increased AQP4 expression in the microvesicular fraction of CSF from patients with obstructive hydrocephalus. CSF, cerebrospinal fluid. S, supernatant; P, pellet; TP, total protein; 4V, tetraventricular hydrocephalus; CONT, control; DW, Dandy Walker malformation; OAS, mesencephalic aqueduct obstruction, POST HEM, post-hemorrhagic hydrocephalus; OBS, obstructive hydrocephalus; COM, communicating hydrocephalus.