Fig. 7From: AQP4 labels a subpopulation of white matter-dependent glial radial cells affected by pediatric hydrocephalus, and its expression increased in glial microvesicles released to the cerebrospinal fluid in obstructive hydrocephalusExpression of AQP4 and GFAP in the micro vesicular fraction of the CSF. a Schematic representation of vesicular extraction. b Expression of AQP4 is specifically found in the P2 and P3 fractions and is absent in the total protein fraction. TTR (14KD) was used as a load control since its expression was found in all fractions, including total protein obtained from supernatant 3. c Expression of GFAP and AQP4 in vesicular fractions from pellet 2 and pellet 3. No expression was found in total protein from supernatant 3. d Graphic representation of the colocalization between AQP4 and GFAP, with few events that present only AQP4 or only GFAP. e Graphic representation of fluorescence intensity showing increased AQP4 expression in the microvesicular fraction of CSF from patients with obstructive hydrocephalus. CSF, cerebrospinal fluid. S, supernatant; P, pellet; TP, total protein; 4V, tetraventricular hydrocephalus; CONT, control; DW, Dandy Walker malformation; OAS, mesencephalic aqueduct obstruction, POST HEM, post-hemorrhagic hydrocephalus; OBS, obstructive hydrocephalus; COM, communicating hydrocephalus.Back to article page