Fig. 2
AQP4 immunoreactivity in control samples at 20, 21 and 25 PCW. a Panoramic view of a coronal section from a 20 PCW brain. AQP4 expression has expanded to the entire fornix (arrow) and the lateral ganglionic eminence (arrowhead). b Panoramic view of a coronal slice of 21 PCW. Arrowhead indicates boundary between lateral ganglionic eminence and cortex. c Magnification of the square in B. Arrow indicates specific staining of the subventricular zone of the CC, with decreasing intensity from dorsomedial to dorsolateral. d Magnification of the corpus callosum and basal ganglia. Arrow indicates penetration of the corpus callosum by cellular processes. Arrowhead indicates AQP4 expression at the limit between the lateral and medial ganglionic eminence. e Magnification of the ventromedial zone of the globus pallidum and the internal capsule. Arrow indicates a cellular process penetrating into the capsular fibers. f Coronal section image of the lateral parieto-occipital neocortex at 25 PCW. g, h Magnification of the intermediate zone showing AQP4 immunoreactivity in astrocyte bodies (arrow) and astrocyte end-feet surrounding the blood vessels forming the neurovascular unit (arrowheads). i, j Detail of the outer and inner subventricular zone of the lateral parieto-occipital neocortex. Arrows indicate immunoreactive radial processes and arrowheads indicate cellular bodies. k Coronal section image at a central level showing ganglionic eminences and corpus callosum. Arrows indicate immunoreactive processes that do no progress radially but follow the corpus callosum fibers in the same plane as the section. l Image of the lenticular nucleus (putamen and globus pallidum). Arrows indicate cellular processes extending toward the internal capsule. Ca, caudate; CC, corpus callosum; CGE, caudal ganglionic eminence; CP, cortical plate; EC, external capsule; Fo, fornix; IC, internal capsule; ISVZ, inner subventricular zone; IZ, intermediate zone (subplate); LGE, lateral ganglionic eminence; LV, lateral ventricle; MGE, medial ganglionic eminence; OSVZ, outer subventricular zone; Pa, pallidum; PM, pia mater; Pu, putamen; Tha, thalamus; IC, Isocortex; VHip, ventral hippocampus; VZ, ventricular zone. Scale bars: a 250; b 250 µm; c–e 90 µm; f 320 µm; g, i, j 240 µm; h 80 µm; k, l 500 µm