Fig. 6
Validation of ARL6IP1 interaction with lipid transport proteins. a Representative confocal images showing colocalisation between endogenous FLAG-tagged ARL6IP1 and EGFP-ORP8 in U-2 OS cells. b Western blot analysis of FLAG M2 affinity co-immunoprecipitation showed that in ARL6IP1-FLAG expressing cells EGFP-ORP8 but not EGFP co-immunoprecipitates with ARL6IP1-FLAG. c Representative confocal images of BODIPY stained LDs (green) in posterior axon bundles from flies expressing Sec61β-tomato (magenta; arrowheads indicate punctate or disrupted staining) under the control of the neuronal driver n-SybGAL4. Graphs represent the mean ± SEM number of LDs per μm2 d and the proportion of axon bundles with continuous Sec61β-tomato staining e in the genotypes indicated. n = 21–24 animals per genotype. Statistics consist of one-way ANOVA with Bonferroni’s post-hoc tests