Fig. 3
Within long motor neurons, neuronal loss of Arl6IP1 induces cell non-autonomous LD accumulation in glia. a Representative confocal images of BODIPY stained LDs (green) in axon bundles from flies expressing Sec61β-tomato (magenta) under the control of the indicated neuronal and glial drivers. LDs co-localise with Sec61β-tomato expressed in glia (arrows) but not neurons (arrowheads). b and d Representative confocal images of BODIPY-stained LDs (green) within posterior axon bundles b neuronal membranes labelled with HRP, magenta). c and e Graphs represent the mean ± SEM number of LDs per μm2 in the genotypes indicated. n = 18—35 animals per genotype. Statistics consist of c one-way ANOVA with Dunnett’s and e two-way ANOVA with Bonferroni’s multiple comparisons test. f Locomotor analysis of flies generated by crossing w1118 (control) or Arl6IP1 RNAi to n-SybGAL4, RepoGAL4 or Nrv2GAL4 as indicated. Graphs represent mean ± SEM percentage of flies that reach the top of the vial within 15 s. n = 10 – 13 independent experiments per genotype. Statistics consist of two-way ANOVA with Bonferroni post-hoc test