Fig. 2

Loss of Arl6IP1 disrupts ER and mitochondrial organisation within long, but not short, motor neurons in vivo. a Representative confocal images of the smooth ER marker Rtnl1::YFP (green) within anterior and posterior NMJs (DLG, magenta). b Graph represents mean ± SEM proportion of continuous Rtnl1::YFP staining identified in CRISPR control and Arl6IP1 knockout larvae. n = 19–42 animals. Statistics consist of two-way ANOVA with Tukey’s multiple comparisons test. c Representative confocal images of mitochondria (green) within anterior and posterior axon bundles. Graphs represent mean ± SEM mitochondrial circularity d and size e within axon bundles in Arl6IP1 KO flies compared to controls. n = 18—20 animals per genotype. Statistics consist of two-way ANOVA with Tukey’s multiple comparisons test