Fig. 5
PTau and pSyn loads are strongly correlated across hippocampal subregions and conditions. The Spearman correlation bubble charts (log2 scale) display a strong positive correlation between pTau and pSyn loads across a conditions and subfields (R2Spear = 0.79) and b at subregional level: CA1 (R2Spear = 0.75), CA3 (R2Spear = 0.79) and DG/CA4 (R2Spear = 0.78). Corresponding Aβ loads are labeled by the size of the bubbles. Lower but positive correlation are observed between pTau and Aβ (R2Spear = 0.30) and between pSyn and Aβ (R2Spear = 0.26) across the three subregions. c The correlation between pTau and pSyn is also visible at cellular level with mainly mono-positive neurons for one or the other type of inclusion, but also some rare double-positive neurons that contain pTau and pSyn inclusions at the same time. CA1 from an 82-year-old male AD patient (case 22) immunostained for pSyn (mouse, 11A5, green), pTau (rabbit, PSer422, Thermofisher 44-764G, magenta) and fluorescent DNA dye DRAQ7™ (blue). Rare pyramidal neurons that co-express pSyn and pTau are indicated by full arrowheads. More commonly, pyramidal neurons are positive for either pTau (full triangles) or pSyn (empty triangles) inclusions. To facilitate visualization of mono- respectively double-positive neurons, the location of arrows and triangles is kept in all staining images. Nuclei of all cells are stained in blue. d Colocalization (arrowheads) of AT8 (mouse, cyan) and PSer422 (rabbit, magenta) is confirmed in the CA1 of a 90-year-old AD patient (case 28). Scale bars c, d 30 μm