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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Microglia phenotypes are associated with subregional patterns of concomitant tau, amyloid-β and α-synuclein pathologies in the hippocampus of patients with Alzheimer’s disease and dementia with Lewy bodies

Fig. 4

PTau, Aβ and pSyn loads show distinct disease-associated, subregional patterns in the hippocampus of AD and DLB patients. PTau, Aβ, and pSyn burdens were assessed via immunofluorescence staining (big panels and zoom in small upper panels) and volumetric analysis in CA1, CA3 and DG/CA4 of age-matched CTLs, AD and DLB patients. The staining signal was segmented (with Imaris 9.6) (small lower panels) and their volume measured from 3D acquisitions of each hippocampal subfield for each sample. a PHFs and NFTs of pTau (cyan) were stained with anti-AT8, example of an 80-year-old male AD patient (case 21). b Aβ plaques and subcellular inclusions (magenta) were stained with anti-4G8, example of a 90-year-old male AD patient (case 30). c PSyn inclusions, Lewy bodies and Lewy neurites (green) were stained with anti-11A5, example of a 64-year-old male DLB patient (case 15). Violin plots (with box plots) show that d PTau levels are significantly higher in the AD samples as compared to both DLB and CTLs in all three hippocampal subfields, but a significant increase was also observed in DLB samples compared to CTLs. e Aβ levels are significantly higher in AD samples in all three subfields compared to CTLs, whereas no significant differences are seen between DLB and both AD and CTLs. Aβ loads follow the same subregional pattern in DLB but with lower values. f PSyn loads are significantly increased in AD in all subfields, and in CA1 and CA3 in DLB compared to age-matched controls. PSyn volumes in all subfields are also significantly higher in AD samples compared to DLB. Wilcoxon–Mann–Whitney U-test P values are indicated in the graphs: *P < 0.05; **P < 0.01 and ***P < 0.001. Scale bars in ac big panels = 200 µm, small panels = 30 µm

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