Fig. 1From: Microglia phenotypes are associated with subregional patterns of concomitant tau, amyloid-β and α-synuclein pathologies in the hippocampus of patients with Alzheimer’s disease and dementia with Lewy bodiesMIC-MAC 2.0 analysis workflow. MIC-MAC 2.0 pipeline with illustrations of acquisition (a), automated image processing (b), manual validation via overlay (c), feature extraction (d), feature analysis (e) and cluster analysis (f). a–c Original Iba1 staining of microglia is represented in white, segmented microglia cells in magenta. d Microglia 3D geometrical features are based on nodes (connection points) and edges (branches). f Spatial cluster colour coded overlay. Scale bars in a–c, f = 100 µmBack to article page