Fig. 3

GSEA ranking and validation of targets identified by RNA-sequencing in EZH2 LOF tumors. a. Hallmark positive enrichment for ‘E2F targets’, ‘G2M checkpoint’ and ‘interferon gamma response’ (top) and negative enrichment for ‘oxidative phosphorylation’ (bottom) bar plots are shown. b. Volcano plot showing the enrichment of genes in the EZH2 LOF tumors, Psmb9 is highlighted. c. Validation of RNA-seq targets by qRT-PCR (top, p < 0.05, unpaired t-test, n = 3 tumors/group) and western blot (bottom) shows significant expression of immunoproteasome genes (Psmb9 fold change by qPCR and immunoblot = 2.5). d. Representative staining of EZH2 WT and EZH2 LOF tumors for Psmb9 (top) and human DMG samples for Psmb9, 8 and 10 (bottom). Scale bars are 20 µm