Fig. 3

Patterns of ddPCR-based SNP analyses for loss of heterozygosity on chromosome 10 in 13 control blood samples and 22 selected tumor samples, previously demonstrated to have losses on 10q only (tumor samples 1- 3), no losses on 10 (tumor sample 4), or losses on both 10p and 10q (tumor samples 5–22). White rectangles, non-informative SNP; light grey rectangles, informative SNP with retained heterozygosity; black rectangles, informative SNP with loss of heterozygosity. Tumor sample 1, astrocytoma, IDH-mutant, CNS WHO grade 2; Tumor sample 2, astrocytoma, IDH-mutant, CNS WHO grade 4; Tumor samples 3–22, glioblastoma, IDH-wildtype, CNS WHO grade 4. The majority of samples showed at least one informative (heterozygous) SNP on each chromosomal arm, except for 3 samples that were not informative at the SNPs on 10p, one sample that was not informative at the SNPs on 10q, and one sample that was not informative at the 8 SNPs at both chromosomal arms. Presence or absence chromosome 10 losses in the individual tumor cases were independently validated by other methods (see text)