Fig. 6

Distinct lysosomal phenotypes among different types of neurons in the cortex (CTX) in young Tmem106b^−/− mice. a, b Immunostaining of Calbindin and Cath D in brain sections from 5-month‐old WT and Tmem106b^−/− mice, and images were captured from the frontal cortex. The intensity of Cath D in Calbindin-positive neurons was quantified in b. n = 3. **, p < 0.01, unpaired t-test. Scale bar = 10 μm. c, d Immunostaining of PVALB and Cathepsin D (Cath D) in brain sections from 5-month‐old WT and Tmem106b^−/− mice. Representative images from the frontal cortex are shown. The intensity of Cath D in PVALB-positive interneurons was quantified in d. n = 3. **, p < 0.01, unpaired t-test. Scale bar = 10 μm. e, f Immunostaining of CUX1, Cath D, and NeuN in brain sections from 5-month‐old WT and Tmem106b^−/− mice. Representative images from the frontal cortex are shown. The intensity of Cath D in CUX1-positive excitatory neuron was quantified in f. n = 3. ****, p < 0.0001, unpaired t-test. Scale bar = 10 μm