Fig. 5

Distinct lysosomal phenotypes among different types of neurons in the cerebellum of young Tmem106b^−/− mice. a, b Immunostaining of PVALB and Cathepsin D (Cath D) in cerebellar sections from 2-month‐old WT and Tmem106b^−/− mice. Representative images from the molecular cell layer are shown. The intensity of Cath D in PVALB-positive interneurons was quantified in b. n = 3. ****, p < 0.0001, unpaired t-test. Scale bar = 10 μm. c, d Immunostaining of Cathepsin D (Cath D) and Hoechst in cerebellar sections from 2-month‐old WT and Tmem106b^−/− mice, and images were captured from granule cell layer. The intensity of Cath D in the granule cell layer was quantified in d. n = 3. **, p < 0.001, unpaired t-test. Scale bar = 10 μm. e, f Immunostaining of Cathepsin D (Cath D) and MAP2 in cerebellar sections from 2-month‐old WT and Tmem106b^−/− mice. Representative images from the DCN region are shown. The intensity of Cath D in MAP2-positive DCN neurons was quantified in f. Zoom-in image shows the enlarged lysosomes in 2-month‐old Tmem106b^−/− mice compared with WT mice. n = 3. ****, p < 0.0001, unpaired t-test. Scale bar = 10 μm