Skip to main content
Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Hornerin deposits in neuronal intranuclear inclusion disease: direct identification of proteins with compositionally biased regions in inclusions

Fig. 4

The NII-rich fraction was isolated from NIID and the control brains. a P1 and P4 fractions of NIID and the control brains were immunostained with the anti-ubiquitin antibody. Green signals indicate the autofluorescence of NIIs, and red signals indicate ubiquitin positive molecules. DAPI signals are observed as blue. Overlapping autofluorescence and ubiquitin positive signals indicating NIIs are observed as yellow in NIID. The autofluorescence observed in the control fractions could be lipofuscin, which does not overlap with ubiquitin positive signals. Scale bars = 100 µm. b Autofluorescence was observed in the SDS-insoluble fractions obtained from each brain sample. Scale bars = 100 µm. c P1, SDS-soluble (SDS) and FA-treated fraction (FA) were immunoblotted with the anti-ubiquitin antibody

Back to article page