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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Hornerin deposits in neuronal intranuclear inclusion disease: direct identification of proteins with compositionally biased regions in inclusions

Fig. 1

The NII-rich fraction was isolated from HD190QG mouse brains. a HD190QG mouse brains were subjected to nuclei fractionation. After the 2% SDS/10 mM DTT treatment, the SDS-insoluble fraction (SDS-insoluble) and the SDS-soluble fraction (SDS) were obtained. By treating the SDS-insoluble fraction with 70% FA, NIIs was obtained and used for further experiments (FA). b Phase-contrast microscopy of P1 and P4 fractions stained with DAPI. Scale bars = 50 µm. c IF staining of the P1 and P4 fractions stained with specific antibodies to GFP and huntingtin (EM48). Scale bars = 100 µm. d Western blotting of the P1, SDS and FA fractions stained with specific antibodies to ubiquitin, GFP and huntingtin (EM48). The broad bands indicated by arrowheads are nHTT-EGFP. TG refers to HD190QG transgenic mouse samples, and WT refers to wild type

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