Fig. 3

MLKL ablation ameliorates neurodegeneration and memory loss induced by Aβo in mice. A, B Representative micrographs and quantitative analysis of brain sections from wild-type mice subjected to intracerebroventricular infusion of Aβo or vehicle at 18 days post-injection, immunostained with an Iba1 specific antibody (scale bar, 150 μm). C, D Representative images and quantitative analysis of brain sections immunolabeled with an anti-non-phosphorylated neurofilament antibody at 18 days post Aβo injection (scale bar, 150 μm). E, F Representative images and quantitative analysis of brain sections immunolabeled with an anti-pMLKL antibody at 18 days post Aβo injection (scale bar, 150 μm). Association analyses of (G) pMLKL and Fluoro-Jade C stained area and H pMLKL stained area and cognitive performance, were done by linear regression. I, J Representative micrographs and quantitative measurement of brain sections from mice, treated as indicated in the images at 18 days post Vehicle or Aβo injection, stained with Fluoro-Jade C (scale bar, 150 μm). n = 13 per group, 4 sections per brain, one image of the hippocampus per section was analyzed. K, L, N, O The graphs represent quantitative analyzes of the indicated parameters, obtained from the MWM test. M The heat map shows a graphic representation of the time that mice, treated as indicated in the image, spent in the target quadrant (TQ) during the MWM test. Data are presented as mean ± S.E.M. The data in B, D, and F were analyzed by Student’s t-test. Data in G and H were analyzed by linear regression using Pearson correlation. Data in J-L, N, and O were analyzed by one-way ANOVA followed by Bonferroni post-test. *p < 0.05; **p < 0.01; ***p < 0.001