Fig. 1

Aβ deposits associate with pMLKL in AD brains. A Representative pictures of the localization of pMLKL in relation to the thioflavin S-positive Aβ deposits in hippocampal brain areas of AD (n = 12), MCI, (n = 3), and CTRL (n = 4) patients (scale bar, CTRL1 25 μm; CTRL2 25 μm; MCI1 50 μm; MCI2 25 μm; AD1 100 μm; and AD2 25 μm). B Representative micrographs of the localization of pMLKL in relation to the Amylo-Glo-positive Aβ deposits in hippocampal brain areas of AD (n = 3) patients (scale bar, 100 μm). C Representative images of hippocampal areas of human AD brains (n = 3), stained with the Amylo-Glo Aβ marker (blue), and co-labeled with the indicated antibodies (scale bar, 25 μm). D, E Colocalization analyses between MAP2 (green) and pMLKL (red, left panel), and between Iba1 (green) and pMLKL (red, right panel) in hippocampal areas of human AD brains (n = 3) was done by determining the Manders coefficient (scale bar, 100 μm). Data are presented as mean ± S.E.M. Data in E were analyzed by Student’s t-test. **p < 0.01