Fig. 8
Pathologic α-synuclein colocalizes with neurons, oligodendrocytes, and microglia in the CNS of diseased TgM83+/− mice. Immunofluorescence staining of tissue sections of the brain stem show that phosphorylated α-synuclein (pSyn#64, red) colocalizes with neurons (NeuN, green) as well as with oligodendrocytes (Olig2, green) in mice 360 days after MCAO as indicated by yellow arrows. Deposits of phosphorylated α-synuclein (pSyn#64, red) were additionally seen to colocalize with microglia (Iba1, white) but not with astrocytes (GFAP, white) as indicated by yellow arrows. Neurons, oligodendrocytes, microglia, or astrocytes of mice that underwent sham-surgery, for which only merged images are shown, did not accumulate any phosphorylated α-synuclein deposits. Nuclear staining with DAPI is shown in blue. The scale bar represents 10 µm