Fig. 6

Diseased TgM83^+/− mice display various types of pathologic α-synuclein deposits in the CNS. Immunohistochemical staining of coronal brain tissues sections of diseased TgM83^+/− mice revealed abundant deposits of phosphorylated α-synuclein throughout the brain at 360 days after stroke using the pSyn#64 (a–f), EP1536Y (h), and 81A (j) antibodies that recognize phosphorylated α-synuclein at Ser129, which is associated with the accumulation of pathologic α-synuclein. Deposits of phosphorylated α-synuclein were mostly cytosolic (b–d), sometimes perinuclear (e), or dendritic (arrow) (f). In contrast, sham-treated mice did not show any deposits of phosphorylated α-synuclein with any of the three antibodies used (g, i, k). Staining with the 81A antibody resulted in a higher background due to a known cross reactivity of this antibody with phosphorylated neurofilament subunit L (j, k). The scale bars represent 20 µm