Fig. 4

MCAO-treated TgM83^+/− mice display a late loss of dopaminergic neurons in the substantia nigra. Immunhistochemical analysis of the substantia nigra of MCAO- and sham-treated TgM83^+/− mice with an antibody against TH showed an ipsilateral loss of dopaminergic neurons 360 days after MCAO (a). Quantification of TH-positive cells revealed a significant loss of dopaminergic neurons on the ipsilateral side of the substantia nigra of TgM83^+/− mice after MCAO-treatment (dark red) relative to the contralateral side (light red) or to sham-treated control animals (dark and light blue) at 360 days post surgery (b). The data represent the mean count of TH-positive cells per coronal section of the substantia nigra ± standard deviation. Four coronal brain sections of three to five animals were analyzed per group 14, 30, 90, 180, and 360 days post surgery. P values were computed using two-way ANOVA followed by Tukey’s post-hoc test (P: ** < 0.01, *** < 0.001). The scale bar represents 0.5 mm