Fig. 3

Cellular localization of Adora3 mRNA expression in the brain after cisplatin treatment. a, b Expression of Adora3 mRNA in neurons, microglia, astrocytes, and oligodendrocytes in the cortex of control mice. RNAscope in situ hybridization of Adora3 (white), Map2 (neuron: red) and Tmem119 (microglia: green) (a); Adora3 (white), Gfap (astrocytes: red) and Olig2 (Oligodendrocytes: green) (b) as analyzed by confocal microscopy; scale bar 10 µm. c–g Influence of cisplatin on cellular expression of Adora3, 72 h after the first round of cisplatin treatment; scale bar 50 µm. c Percentage of Adora3 positive neurons (d), microglia (e), astrocytes (f), and oligodendrocytes (g) in the cortex (n = 3). Data are expressed as mean ± SEM and were analyzed with non-parametric Mann-Witney test; *p < 0.05