Fig. 3

Increased insolubility of FUS in the FTLD patient’s brain compared to controls a Western blot analysis of proteins extracted from frozen brain tissue, from controls (CTL-1, -2) or affected patient (AH-11–02). FUS staining was performed on samples resulting from sequential brain extraction, using buffers with increasing solubilisation properties. Total protein was used as loading control with Stain-Free technology (SF). Three independent extractions per individual were performed. Representative blots and Stain-Free staining are shown. b Graph representing the ratio of FUS bands intensity in the soluble fraction (HS—HS-Tx—RIPA) relative to the insoluble fractions (2%SDS—Ac.Form) after SF normalization [means ± standard error of the mean (SEM)]