Fig. 2

Effects of homo- and heterozygous C215Y mutation on brain morphology. A–C Hematoxylin and eosin staining of coronal brain sections from C215Y/ + , C215Y/C215Y, and wt (+ / +) littermates at the embryonic day 18.5 (A) reveals insignificant changes in the overall brain area (B) but enlarged lateral ventricles in the mutants (C). D–F Coronal sections through the motor cortex (D) show differences in the cortical thickness between wt and C215Y/C215Y mice (E). Quantification of the cell density in this region reveals that it is decreased in the two mutant genotypes (F). Data are presented as mean ± SD, n = 3–5 animals. *p < 0.05, **p < 0.01, ***p < 0.005, significance from the wt is assessed by t-test. Scale bar: 50 µm