Fig. 1

Survival rate of F0 mice of different genotypes after transgenesis. A G203R F0 mice, either homozygous or heterozygous, reveal dramatic postnatal lethality. Only two G203R/ + mice lived past postnatal day 1. B In contrast, heterozygous or even homozygous F0 G215Y mice revealed the survival not different from fully wt mice (dotted line). GNAO1^−/− loss-of-function animals generated as a by-product of the transgenesis showed reduced survival. “−” designates a truncated allele resulting from CRISPR-mediated indels