Table 1 Overview of the primary antibodies used in the present study
Antibody | Fluorophore | Clone | Species | Dilution | Source | Product number | |
|---|---|---|---|---|---|---|---|
Tissue | In vitro | ||||||
IHQ | FC | ||||||
CD45 | BV425 | 30-F11 | Rat monoclonal | Â | 1:200 | BioLegend | 103134 |
CD3É› | AF700 | 500-A2 | Armenian Hamster monoclonal | Â | 1:100 | BioLegend | 100320 |
CD11b | BV510 | M1/70 | Rat monoclonal | Â | 1:500 | BioLegend | 101263 |
MHC-II | PerCP-710 | AF6-120.1 | Mouse monoclonal | Â | 1:200 | eBioscience | 46-5320-80 |
TLR4 | PE-Cy7 | SA15-21 | Rat monoclonal | Â | 1:250 | BioLegend | 145407 |
Ly6C | BV785 | HK1.4 | Rat monoclonal | Â | 1:2000 | BioLegend | 128041 |
Ly6G | AF700 | 1A8 | Rat monoclonal | Â | 1:100 | BioLegend | 127622 |
NeuN | Â | A60 | Rabbit polyclonal | 1:4000 | Â | Millipore | MAB377 |
Iba1 | Â | NCNP24 | Rabbit polyclonal | 1:1000 | Â | Wako | 019-19741 |
CD68 | Â | Gp110 | Rabbit polyclonal | 1:1000 | Â | Invitrogen | PA5-32330 |
PDGFR-α |  |  | Goat polyclonal | 1:250 |  | R & D systems | AF1062 |
PSD-95 | Â | Â | Rabbit polyclonal | 1:200 | Â | Invitrogen | 51-6900 |
Synapsin | Â | Â | Guinea Pig | 1:1000 | Â | Synaptic systems | 106 004 |
Secondary antibody | AF488 | Â | Goat anti-rabbit | 1:500 | Â | Thermo Fisher | A-32731 |
Secondary antibody | AF568 | Â | Goat anti-guinea pig | 1:500 | Â | Thermo Fisher | A-11073 |
Secondary antibody | AF568 | Â | Donkey anti-goat | 1:500 | Â | Thermo Fisher | A-11055 |
Secondary antibody | AF488 | Â | Donkey anti-rabbit | 1:500 | Â | Thermo Fisher | A-21206 |
Secondary antibody | Â | Â | Biotinylated goat anti-rabbit | 1:1000 | Â | Vector Laboratories | BA-1000-1.5 |