Skip to main content
Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Immediate induction of varicosities by transverse compression but not uniaxial stretch in axon mechanosensation

Fig. 5

Transvers compression rapidly and reversibly induced axonal varicosity formation in cultured cortical neurons in the fluid puffing assay. A Diagram of fluid puffing set up, providing transverse compression. B Initiation and recovery of axonal varicosities in cultured cortical neurons by puffing. Cultured cortical neurons were transfected with YFP cDNA at 6 DIV and imaged at 8–10 DIV. YFP signals are inverted. C Summary of the density of axonal varicosities induced by 150-s puffing. Unpaired t-test: *p < 0.05. n = 7. D Colocalization of YFP (green) and NF staining (red) in control axons (top) and axons with varicosities post puffing (middle and bottom). E Heterogeniety of puffing-induced axonal varicosities revealed by TEM. e1, several varicosities along an axon; e2-e4, mitochondria and multivasicular body in axonal varicosities with several microtubule filaments; e5-e6, amorphies structures with no microtubule filaments in varicosities; e7, extensive and exclusive vesicular structure within the varicosity. F Diagram of the cross section of an axon with the loading and the boundary condition in the simulation. G The maximum component of the first principal strain under different pressures in the simulation with finite element analysis. The strain level is indicated by color. Scale bars, 30 μm in B, 15 μm in D, and 2 μm in E

Back to article page